Spleen cell
Spleen cell have been used to analyse the prescent of SV40 T antigen (T Ag) CD8 T cell,which have been predicted in vivo by bioluminescence imaging. Therefore for a series of transgenic mice which express a bioluminescent antigenic fusion protein which enables non-invasive monitoring of SV40 Large T fused to firefly luciferase have been generated. the fusion protein can is expresses under the control of a conditional expression cassette which can be activated by homologoues recombination. Data show that T cells can be predicted in mice dependent on antigen expression level in single transgenic mice.
Sensitivity of bioluminescent imaging in order to predict T cells could be demonstrated by analysing T cells in Cre-activtated double transgenic mice (see here), which expresses the fusion protein in the liver, because this mice show full transgene activation, which should result in full negative selection of T-Ag specific T cells and full bioluminescent signal intensity.
Resources
- Buschow C, Charo J, Anders K, Loddenkemper C, Jukica A, Alsamah W, Perez C, Willimsky G, Blankenstein T, 2010 Mar 15. "In vivo imaging of an inducible oncogenic tumor antigen visualizes tumor progression and predicts CTL tolerance." J Immunol 184 (6): 2930-8 [PubMed]
Last modified: 18.05.2025 - 19:32:06
